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nhe3 ab  (Novus Biologicals)


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    Novus Biologicals nhe3 ab
    Nhe3 Ab, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhe3 ab/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    nhe3 ab - by Bioz Stars, 2026-02
    90/100 stars

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    Effects of EX4 and ALG on phosphorylated Na+/H+ exchanger (NHE3) in renal membranes and plasma dipeptidyl peptidase-4 (DPP-4) activity. Kidneys and plasma were harvested 1 h after application of EX4 (10 μg/kg body wt ip), ALG (10 mg/kg body wt ip), or vehicle (0.85% NaCl, 2 μl/g body wt). A–C: natriuretic response to EX4, but not ALG, was associated with increased NHE3 phosphorylated at S552 (pS552) and S605 (pS605) in renal membranes. This effect of EX4 was absent in Glp1r−/− mice. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle. D: ALG inhibited DPP-4 activity in WT and Glp1r−/− mice. Data are expressed relative to the mean of the vehicle-treated WT group, which was set as 100%. EX4 was without effect. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice

    doi: 10.1152/ajprenal.00259.2012

    Figure Lengend Snippet: Effects of EX4 and ALG on phosphorylated Na+/H+ exchanger (NHE3) in renal membranes and plasma dipeptidyl peptidase-4 (DPP-4) activity. Kidneys and plasma were harvested 1 h after application of EX4 (10 μg/kg body wt ip), ALG (10 mg/kg body wt ip), or vehicle (0.85% NaCl, 2 μl/g body wt). A–C: natriuretic response to EX4, but not ALG, was associated with increased NHE3 phosphorylated at S552 (pS552) and S605 (pS605) in renal membranes. This effect of EX4 was absent in Glp1r−/− mice. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle. D: ALG inhibited DPP-4 activity in WT and Glp1r−/− mice. Data are expressed relative to the mean of the vehicle-treated WT group, which was set as 100%. EX4 was without effect. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle.

    Article Snippet: Immunoblotting was performed at 4°C overnight with the primary NHE3 Ab (Millipore, Billerica, MA), phosphorylated (S552) NHE3 Ab (Novus Biologicals, Littleton, CO), and phosphorylated (S605) NHE3 Ab (Santa Cruz Biotechnology, Santa Cruz, CA) diluted 1:1,000, 1:1,000, and 1:200, respectively.

    Techniques: Clinical Proteomics, Activity Assay

    Natriuresis and increase in NHE3 phosphorylation in response to EX4 are preserved in awake adenylyl cyclase type VI (AC6)-deficient (AC6−/−) mice. A: AC6−/− and WT mice were randomized to application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt) followed by oral gavage with isotonic saline (30 μl/g; ∼30% of daily NaCl intake), and urinary excretion in metabolic cages over 3 h was determined. Natriuretic effect of EX4 was similar in both genotypes. EX4 did not change urinary cAMP excretion. Values are means ± SE; n = 5–6 per group. *P < 0.05 vs. vehicle. B: kidneys were harvested from AC6−/− mice 1 h after application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt). Phosphorylation of renal NHE3 at S552 and S605 was preserved in AC6−/− mice. Values are means ± SE; n = 6 per group. *P < 0.05 vs. vehicle.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice

    doi: 10.1152/ajprenal.00259.2012

    Figure Lengend Snippet: Natriuresis and increase in NHE3 phosphorylation in response to EX4 are preserved in awake adenylyl cyclase type VI (AC6)-deficient (AC6−/−) mice. A: AC6−/− and WT mice were randomized to application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt) followed by oral gavage with isotonic saline (30 μl/g; ∼30% of daily NaCl intake), and urinary excretion in metabolic cages over 3 h was determined. Natriuretic effect of EX4 was similar in both genotypes. EX4 did not change urinary cAMP excretion. Values are means ± SE; n = 5–6 per group. *P < 0.05 vs. vehicle. B: kidneys were harvested from AC6−/− mice 1 h after application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt). Phosphorylation of renal NHE3 at S552 and S605 was preserved in AC6−/− mice. Values are means ± SE; n = 6 per group. *P < 0.05 vs. vehicle.

    Article Snippet: Immunoblotting was performed at 4°C overnight with the primary NHE3 Ab (Millipore, Billerica, MA), phosphorylated (S552) NHE3 Ab (Novus Biologicals, Littleton, CO), and phosphorylated (S605) NHE3 Ab (Santa Cruz Biotechnology, Santa Cruz, CA) diluted 1:1,000, 1:1,000, and 1:200, respectively.

    Techniques: Phospho-proteomics, Saline

    Effects of EX4 and ALG on phosphorylated Na+/H+ exchanger (NHE3) in renal membranes and plasma dipeptidyl peptidase-4 (DPP-4) activity. Kidneys and plasma were harvested 1 h after application of EX4 (10 μg/kg body wt ip), ALG (10 mg/kg body wt ip), or vehicle (0.85% NaCl, 2 μl/g body wt). A–C: natriuretic response to EX4, but not ALG, was associated with increased NHE3 phosphorylated at S552 (pS552) and S605 (pS605) in renal membranes. This effect of EX4 was absent in Glp1r−/− mice. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle. D: ALG inhibited DPP-4 activity in WT and Glp1r−/− mice. Data are expressed relative to the mean of the vehicle-treated WT group, which was set as 100%. EX4 was without effect. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice

    doi: 10.1152/ajprenal.00259.2012

    Figure Lengend Snippet: Effects of EX4 and ALG on phosphorylated Na+/H+ exchanger (NHE3) in renal membranes and plasma dipeptidyl peptidase-4 (DPP-4) activity. Kidneys and plasma were harvested 1 h after application of EX4 (10 μg/kg body wt ip), ALG (10 mg/kg body wt ip), or vehicle (0.85% NaCl, 2 μl/g body wt). A–C: natriuretic response to EX4, but not ALG, was associated with increased NHE3 phosphorylated at S552 (pS552) and S605 (pS605) in renal membranes. This effect of EX4 was absent in Glp1r−/− mice. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle. D: ALG inhibited DPP-4 activity in WT and Glp1r−/− mice. Data are expressed relative to the mean of the vehicle-treated WT group, which was set as 100%. EX4 was without effect. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle.

    Article Snippet: Immunoblotting was performed at 4°C overnight with the primary NHE3 Ab (Millipore, Billerica, MA), phosphorylated (S552) NHE3 Ab (Novus Biologicals, Littleton, CO), and phosphorylated (S605) NHE3 Ab (Santa Cruz Biotechnology, Santa Cruz, CA) diluted 1:1,000, 1:1,000, and 1:200, respectively.

    Techniques: Clinical Proteomics, Activity Assay

    Natriuresis and increase in NHE3 phosphorylation in response to EX4 are preserved in awake adenylyl cyclase type VI (AC6)-deficient (AC6−/−) mice. A: AC6−/− and WT mice were randomized to application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt) followed by oral gavage with isotonic saline (30 μl/g; ∼30% of daily NaCl intake), and urinary excretion in metabolic cages over 3 h was determined. Natriuretic effect of EX4 was similar in both genotypes. EX4 did not change urinary cAMP excretion. Values are means ± SE; n = 5–6 per group. *P < 0.05 vs. vehicle. B: kidneys were harvested from AC6−/− mice 1 h after application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt). Phosphorylation of renal NHE3 at S552 and S605 was preserved in AC6−/− mice. Values are means ± SE; n = 6 per group. *P < 0.05 vs. vehicle.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice

    doi: 10.1152/ajprenal.00259.2012

    Figure Lengend Snippet: Natriuresis and increase in NHE3 phosphorylation in response to EX4 are preserved in awake adenylyl cyclase type VI (AC6)-deficient (AC6−/−) mice. A: AC6−/− and WT mice were randomized to application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt) followed by oral gavage with isotonic saline (30 μl/g; ∼30% of daily NaCl intake), and urinary excretion in metabolic cages over 3 h was determined. Natriuretic effect of EX4 was similar in both genotypes. EX4 did not change urinary cAMP excretion. Values are means ± SE; n = 5–6 per group. *P < 0.05 vs. vehicle. B: kidneys were harvested from AC6−/− mice 1 h after application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt). Phosphorylation of renal NHE3 at S552 and S605 was preserved in AC6−/− mice. Values are means ± SE; n = 6 per group. *P < 0.05 vs. vehicle.

    Article Snippet: Immunoblotting was performed at 4°C overnight with the primary NHE3 Ab (Millipore, Billerica, MA), phosphorylated (S552) NHE3 Ab (Novus Biologicals, Littleton, CO), and phosphorylated (S605) NHE3 Ab (Santa Cruz Biotechnology, Santa Cruz, CA) diluted 1:1,000, 1:1,000, and 1:200, respectively.

    Techniques: Phospho-proteomics, Saline

    Effects of EX4 and ALG on phosphorylated Na+/H+ exchanger (NHE3) in renal membranes and plasma dipeptidyl peptidase-4 (DPP-4) activity. Kidneys and plasma were harvested 1 h after application of EX4 (10 μg/kg body wt ip), ALG (10 mg/kg body wt ip), or vehicle (0.85% NaCl, 2 μl/g body wt). A–C: natriuretic response to EX4, but not ALG, was associated with increased NHE3 phosphorylated at S552 (pS552) and S605 (pS605) in renal membranes. This effect of EX4 was absent in Glp1r−/− mice. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle. D: ALG inhibited DPP-4 activity in WT and Glp1r−/− mice. Data are expressed relative to the mean of the vehicle-treated WT group, which was set as 100%. EX4 was without effect. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice

    doi: 10.1152/ajprenal.00259.2012

    Figure Lengend Snippet: Effects of EX4 and ALG on phosphorylated Na+/H+ exchanger (NHE3) in renal membranes and plasma dipeptidyl peptidase-4 (DPP-4) activity. Kidneys and plasma were harvested 1 h after application of EX4 (10 μg/kg body wt ip), ALG (10 mg/kg body wt ip), or vehicle (0.85% NaCl, 2 μl/g body wt). A–C: natriuretic response to EX4, but not ALG, was associated with increased NHE3 phosphorylated at S552 (pS552) and S605 (pS605) in renal membranes. This effect of EX4 was absent in Glp1r−/− mice. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle. D: ALG inhibited DPP-4 activity in WT and Glp1r−/− mice. Data are expressed relative to the mean of the vehicle-treated WT group, which was set as 100%. EX4 was without effect. Values are means ± SE; n = 5 per group. *P < 0.05 vs. vehicle.

    Article Snippet: Immunoblotting was performed at 4°C overnight with the primary NHE3 Ab (Millipore, Billerica, MA), phosphorylated (S552) NHE3 Ab (Novus Biologicals, Littleton, CO), and phosphorylated (S605) NHE3 Ab (Santa Cruz Biotechnology, Santa Cruz, CA) diluted 1:1,000, 1:1,000, and 1:200, respectively.

    Techniques: Clinical Proteomics, Activity Assay

    Natriuresis and increase in NHE3 phosphorylation in response to EX4 are preserved in awake adenylyl cyclase type VI (AC6)-deficient (AC6−/−) mice. A: AC6−/− and WT mice were randomized to application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt) followed by oral gavage with isotonic saline (30 μl/g; ∼30% of daily NaCl intake), and urinary excretion in metabolic cages over 3 h was determined. Natriuretic effect of EX4 was similar in both genotypes. EX4 did not change urinary cAMP excretion. Values are means ± SE; n = 5–6 per group. *P < 0.05 vs. vehicle. B: kidneys were harvested from AC6−/− mice 1 h after application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt). Phosphorylation of renal NHE3 at S552 and S605 was preserved in AC6−/− mice. Values are means ± SE; n = 6 per group. *P < 0.05 vs. vehicle.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice

    doi: 10.1152/ajprenal.00259.2012

    Figure Lengend Snippet: Natriuresis and increase in NHE3 phosphorylation in response to EX4 are preserved in awake adenylyl cyclase type VI (AC6)-deficient (AC6−/−) mice. A: AC6−/− and WT mice were randomized to application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt) followed by oral gavage with isotonic saline (30 μl/g; ∼30% of daily NaCl intake), and urinary excretion in metabolic cages over 3 h was determined. Natriuretic effect of EX4 was similar in both genotypes. EX4 did not change urinary cAMP excretion. Values are means ± SE; n = 5–6 per group. *P < 0.05 vs. vehicle. B: kidneys were harvested from AC6−/− mice 1 h after application of EX4 (10 μg/kg body wt ip) or vehicle (0.85% NaCl, 2 μl/g body wt). Phosphorylation of renal NHE3 at S552 and S605 was preserved in AC6−/− mice. Values are means ± SE; n = 6 per group. *P < 0.05 vs. vehicle.

    Article Snippet: Immunoblotting was performed at 4°C overnight with the primary NHE3 Ab (Millipore, Billerica, MA), phosphorylated (S552) NHE3 Ab (Novus Biologicals, Littleton, CO), and phosphorylated (S605) NHE3 Ab (Santa Cruz Biotechnology, Santa Cruz, CA) diluted 1:1,000, 1:1,000, and 1:200, respectively.

    Techniques: Phospho-proteomics, Saline